How do invadopodia disassemble?2018-02-06T10:46:10+08:30

How do invadopodia disassemble?

The final step in function of invadopodia is disassembly which primarily involves dismantling the actin core [1]. Several proteins have been implicated in a cascade leading to this, including paxillin, extracellular signal-regulated kinases (Erk) and calpain [1]. The phosphorylation state of tyrosine residues within paxillin localized to invadopodia controls the rate of disassembly. The mutation of specific tyrosine residues in paxillin render these sites non-phosphorylatable and this results in a significant delay in the disassembly of invadopodia. Furthermore, phosphorylation of paxillin was shown to promote the activation of Erk, which promotes the activation of calpain [1]. Calpains are calcium dependent, non-lysosomal, cysteine proteases. The activation of calpain 2 has been shown to be required for the degradation of cortactin, a component of the actin core and this subsequently promotes invadopodia disassembly [2]. The inhibition of calpain [1] or the expression of calpain-resistant cortactin in cells lacking endogenous cortactin [2], results in a decrease in the rate of invadopodia disassembly. This is akin to the role of calpain in the degradation of talin [3] and focal adhesion kinase [4], which has been shown to promote focal adhesion disassembly.

View All

Latest Findings

Protein Info


  1. Badowski C, Pawlak G, Grichine A, Chabadel A, Oddou C, Jurdic P, Pfaff M, Albigès-Rizo C, and Block MR. Paxillin phosphorylation controls invadopodia/podosomes spatiotemporal organization. Mol. Biol. Cell 2007; 19(2):633-45. [PMID: 18045996]
  2. McMaster OG, Du F, French ED, and Schwarcz R. Focal injection of aminooxyacetic acid produces seizures and lesions in rat hippocampus: evidence for mediation by NMDA receptors. Exp. Neurol. 1991; 113(3):378-85. [PMID: 1833221]
  3. Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, and Huttenlocher A. Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 2004; 6(10):977-83. [PMID: 15448700]
  4. Chan KT, Bennin DA, and Huttenlocher A. Regulation of adhesion dynamics by calpain-mediated proteolysis of focal adhesion kinase (FAK). J. Biol. Chem. 2010; 285(15):11418-26. [PMID: 20150423]