This study shows the dynamic and cooperative binding of multiple vinculins to a stretched antiparallel dimer of talin using super resolution microscopy.
These findings were published in Nano Letters in 2016.
Hu X, Jing C, Xu X, Nakazawa N, Cornish VW, Margadant FM, Sheetz MP. 2016. Cooperative Vinculin Binding to Talin Mapped by Time-Resolved Super Resolution Microscopy. Nano Lett. Jul 13; 16(7):4062-8. doi: 10.1021/acs.nanolett.6b00650.
More information on the Sheetz lab.
Figure: 1. Talin monomer is tagged at the N-terminal with GFP and the C-terminal with mCherry 2. In a talin dimer, the separation between the N-termini is 162 ± 44 nm, whereas the C-terminal dimerization domains colocalize 3. Upto 10 vinculins binds the talin dimer when talin is stretched to 180 nm. (* Note that the 3 part of the gigure can have 180nm separation between the two N-termini.